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The levels of target genes in each sample were normalized on the basis of its β-actin content through the formula: Normalized level (NL) = Level target)/Level β-actin) = 2Ct(target)/Level β-actin= 2Ct target)-Ct(β-actin) = 2ΔCt.
All values were normalized on the basis of the Bradford protein assay.
These issues were not very problematic, as the data was normalized on the basis of national LGNIc values with the correct definition derived from UNDP.
Uptake of 3H-2-deoxyglucose was measured over 5 min and the data were normalized on the basis of total cell protein.
Young's moduli and the maximum stress-at-break of the swollen hydrogels were normalized on the basis of their polymer content.
The nonlinear seismic analysis is performed on near-fault ground motions with significant horizontal or vertical components, selected and normalized on the basis of the design hypotheses adopted for the test structure.
In the procedure, the transient current energy values from both sides of the bus were primarily normalized on the basis of transition resistance and fault angle, and then the characteristic value with functions of direction judge and value comparison was obtained by calculating their difference.
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SUV normalizes on the basis of injected dose and patient weight, which is less accurate as compared to TBR that normalizes on the basis of the blood pool activity itself.
However, we normalized each fingerprint on the basis of its length, such that ∥x i ∥=1.
Total fluorescence per well was normalized on the number of cellular pixels/well.
Luciferase signal intensities were normalized for transfection efficiency on the basis of renilla luciferase signal intensities from the co-transfected pGL4-73[hRluc/SV40] plasmid.
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