To control for input in Beclin 1 anti-immunoprecipitates, values used to calculate VPS34 lipid kinase activity were normalized for levels of Beclin 1 determined by densitometric quantification of Beclin 1 western blot bands in anti-Beclin 1 immunoprecipitates.
Compared with baseline values, aleglitazar 0.03 mg/kg per day reduced triglyceride levels by an average of 89% (328 to 36 mg/dL; P = 0.0035 when normalized for baseline levels) and increased high-density lipoprotein cholesterol levels by 125% (46 to 102 mg/dL; P = 0.0007).
mRNA levels were calculated relative to 18S expression and normalized for expression levels of control mice on chow.
Densitometric analysis of Stat3 PCR products, normalized for HPRT levels, showed a reduction of STAT3 mRNA by approximately 50% in NF861 and NF797 compared to NIH3T3 cells (Figure 2D), thus suggesting a down-regulation of Stat3 at the transcriptional level.
CD163+ macrophages in synovium and sCD163 in synovial fluid (SF) (normalized for albumin levels) were assessed by immunohistochemistry and ELISA, respectively, in 26 SpA and 23 RA patients.
Owing to the reported overprediction of the TDx immunoassay, observed and simulated data were normalized for trough levels immediately before RYGB surgery, indicated by the time of 0 months.
Quantitation of multiple experiments by densitometry reproducibly demonstrates that Gab1F446/472/589 is tyrosine phosphorylated following EGF treatment to similar levels when normalized for Gab1 expression levels.
The radioactivity of each sample was normalized for the protein level, which was determined using a Protein Assay Bicinchoninate Kit (Nacalai Tesque).
All protein bands used were within linear range, and normalized for β-actin level in the same membrane.
SUVs were normalized for blood glucose levels in accordance with EANM guidelines [21].
When the enzyme activity of the Tg-S466L mice was normalized for CBS protein levels, the specific activity of p.S466L was found to be almost equivalent (0.6 ± 0.26 µmol/mg/hr) to wild-type CBS in the presence of AdoMet.
