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A Normalized expression rates (mean±s. d). of SLUG (upper), and FKBP51 (lower) mRNA levels.
Normalized expression rates (mean±s. d). of VIM (upper), SLUG (intermediate) and FKBP51 (lower) mRNA levels.
Calculations of the normalized expression rates were performed according to the ΔCt method.
Normalized expression ratio of nox and npx (test) = 2 ΔΔCt.
To obtain the vimentin or eukaryotic translation elongation factor 1 gamma gene expression rate the amount of target genes were normalized to that of the housekeeping gene Glyceraldehyde 3-phosphate dehydrogenase (GAPDH).
For microarrays, averages are over log2 normalized expression intensity.
We performed a Violin plot analysis to visualize the distribution of the normalized expression levels.
All normalized expression data was analyzed using GeneSpring software.
Normalized expression = Actual miRNA count/Total count of clean reads*1000000.
We used the normalized expression values from the database.
Left panel, BAF; right panel, normalized expression.
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