Sentence examples for normalized copy number from inspiring English sources
To gain an approximate comparison across all genes measured, amplification efficiency was assumed identical across all primer sets and target gene expression was normalized to 18S expression using a logarithmic transformation proportional to normalized copy number (log102-ΔCT), where ΔCT is CT target gene) – CT(18S) [ 30].
The results are reported in Table 1 as the log10 of the normalized copy number.
The normalized copy number values were then used to determine the relative quantities (RQ) of individual gene transcripts.
The normalized copy number data obtained from the 227 samples were mapped into the human genome using the Build 18 (NCBI 36) assembly with an annotation file provided by the manufacturer (http://www.chem.agilent.com/).agilent.com/
We calculated the normalized copy number values for each exon in each sample as follows.
Normalized copy number data was segmented using GLAD with default parameters available in GenePattern version 3.3.3 [ 20].
Final HIF1α mRNA concentrations were expressed in normalized copy numbers as previously described [ 14].
RNAi gene enrichment ranking (RIGER) was used to find phenotype-specific, functionally relevant genes from the scale-normalized copy number count data (Luo et al, 2008).
Normalized chromosome copy number clustered around "disomy" although with significant departures from non-integral values evident for some chromosomes (Fig. 1).
Normalized tag copy number was calculated by dividing tag counts for each gene with the total number of tags generated for each library and are presented per one million transcripts.
All samples were obtained with the patients' informed written consent.> -wrap-foot> Alu-normalizeded relative copy number (×10−4), the value is presented median (25% ~ 75% percentile); bMann–Whitney test, P = 0.007 Total RNA was extracted from 30 50 mg of tumor tissue using a commercial RNA isolation kit according to the manufacturer's protocol (Ultrapure RNA Kit, CWBIO, Beijing).
