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Recording of CI and subsequent normalization of the cell index (normalized cell index, NCI) was performed using the RTCA Software 1.2 (Roche).
The fluorescence intensity of a cell was normalized by FIN = (FIC FIBIB) / FIB, where FIN is the normalized cell fluorescence intensity, FIC is the mean fluorescence intensity of the cell, and FIB, the fluorescence intensity of background, is the mean of four fluorescence intensities from four equidistant round domains (of 36π µm2) around the cell at a distance of 24 µm.
Normalized cell indexes were also calculated by the software at a selected normalization time point, which was chosen as the time just before the addition of nanoparticles.
To account for the different proliferation rates under each culture condition, cytokine measurements were normalized to total protein, as quantified via Pierce BCA and phospho-protein measurements were normalized by loading an equal amount of total protein into each assay well and normalized cell lysate loaded into the multiplexed assay to total protein.
(e) Normalized cell number and area (f) of fibroblasts on the surfaces indicated relative to the RGDS-only value; n=3.
(f) Normalized cell number and area (g) of fibroblasts plated on various coated surfaces (all values were normalized relative to alginate, n=5).
Similar(5)
Western blots of immunoprecipitated normalized cell-lysates from untransfected cells (U) or cells transfected with IGF1R-fl (WT) or IGF1R-fl proteins with cysteine substitutions (895C, 898C, or 905C) incubated the presence or absence of IGF1 (bottom).
Following BCD, patients who repopulated with immature B cells normalized iNKT cell numbers and maintained a clinical response to BCDT.
The relative level of GFP expressing cells was normalized to cells from wildtype littermates.
Retained radioactivity in the cells was measured in a gamma counter and normalized to cell count.
Adhesions were normalized to cell number rather than circumference to avoid changes due to cell cycle.
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