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PCR and normalized calculations were repeated in at least three independent samples.
PCR and normalized calculations were repeated in at least three independent samples from each genotype, rendering an averaged estimation (± standard deviation) of target gene dosage in the haploids.
PCR and normalized calculations were repeated in at least three independent samples from each mutant and wild type, rendering an estimation of target gene dosage in the mutant genotype.
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The model bccr, giss and and inmcm have been detected the warm, cold and normal summer temperature from the normalized anomaly calculations (Table 6).
For normalized SHG calculations (i.e. BSHG normalized to collagen I IF), the BSHG and collagen I IF images were thresholded to select for collagen I-positive features and to reduce artifactual effects from non-specific background in either channel, applying the same thresholding standard to images from all experimental groups.
From the normalized anomaly calculations, the POD value of models has been computed.
Comparisons of normalized PPI calculations among the three sensel window sizes for each wheelchair configuration revealed no significant differences.
Based on the normalized anomaly calculation, model bccr has predicted that more number of warm years than the previous periods.
The normalization procedure, the calculations for normalized inner diameter, and the experimental protocol for the femoral artery were described previously [ 25, 32].
For the normalized peak-area calculations, each peak area was normalized by dividing the individual peak area by the total peak area of all peaks for that sample.
These SCN volume estimates were normalized to volume calculations based on Nissl staining, and show no significant difference between the volume prescribed by little SAAS vs. Nissl staining (p>0.95; data not shown).
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