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We try to normalize the variability of sleep".
They recommended the use of C. elegans control miRNAs during the denaturation of samples to normalize the variability that could affect the reaction efficiency [ 21].
The geometric mean of housekeeping gene GAPDH or β-Actin was used as an internal control to normalize the variability in expression levels and were analyzed using the 2 −ΔΔCT method described [ 45].
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PAC effectively normalizes the variability in gene expression levels between samples and, in a single sample, normalizes the variability in signal intensity between probe sets of the same transcript (Fig. 1).
At present, it is possible to normalize the technical variability of the serum/plasma RNA extraction using the C. elegans spiked-in control miRNAs.
They recommended the use of three C. elegans spiked-in control miRNAs during the denaturation of plasma/serum samples to normalize the biological variability that could affect the extraction efficiency.
In the pcVPC, to normalize for the variability in independent variables (dose, time and body weight) in the graphical display of the predictive performance of the model, the observations and simulations were normalized based on the typical population predictions as follows and previously described 16.
To normalize for the variability of luciferase expression in transfected cells, the relative luminescence values in each cell compartment were expressed as a ratio to the total potential luminescence measured on equal aliquots of the same lysed cells with a luciferase assay kit (Promega, Madison, USA) in the presence of excess ATP.
To normalize
To have an opportunity to evaluate inhomogeneity in gene expression variability, it is necessary to normalize this variability to make it independent of the level of gene expression.
The measure has the advantage of being normalized to the variability of dT and dO and therefore it can also be interpreted as a signal-to-noise ratio when discriminating among multiple stimuli.
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CEO of Professional Science Editing for Scientists @ prosciediting.com