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We used the ΔΔCT method to normalize the qPCR data to mitochondrial DNA Real-time PCRs were carried out on the Light Cycler480 (Hoffmann La Roche, Basel, Switzerland)39.
Both methods will normalize the sequence similarity by the sequence length.
(9)–(12) are normalized using the method in Ref. [16].
The IMM method normalizes the libraries without a symmetry trimmed.
Here we report a method (designated the "Percellome" method) for normalizing the expression of mRNA values in biological samples.
Third, normalizing the linkage score (see "Methods") pruned out a small number of links.
This method first normalizes the decision matrix of a MCDM problem.
Two methods for normalizing the data were applied.
For the organ specific analysis, expression data from LD were background corrected with the "rma" method and normalized with the "quantiles" method using the R affy library [25].
The numerical procedure, based on the Messac normalized constraint method, include discrete, continuous and discrete-continuous sets of design variables.
Data normalization was conducted using the normalize.qsline method from the Bioconductor package in R [ 39].
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