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The copy number of MECP2 was normalized to that of FOXP2 to normalize for differences in DNA input.
We further note that it is possible to normalize for differences between sources using quantile normalization (Bolstad et al., 2003).
To normalize for differences in efficiency of sample extraction or cDNA synthesis we used β-actin as housekeeping gene.
This mean prevalence was chosen for Munich to normalize for differences according to location (southern vs. western Germany) and city size (large vs. small).
Since the number of spike-in molecules is theoretically the same across all single-cell libraries, they can be used to calculate scaling factors to normalize for differences in RNA concentration between individual cells.
Beta-2-microglobulin was used to normalize for differences in input cDNA.
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The β-actin gene Arz_9569_T1 of A. resinae ZN1 was served as an internal control to normalize for difference in total RNA quantity.
An empirical Bayes before after procedure was employed to properly account for regression to the mean while normalizing for differences in traffic volume and other factors between the before and after periods.
The analyses presented above were based on morphogenetic observations normalized for differences in photo-thermal time because genotypes did not attain the 6-leaf stage synchronously.
By comparing calculated masses, we have normalized for differences in detection efficiency between the I.S.- and HBV-specific probes.
This normalizes for differences in the sizes of the genome assemblies being compared.
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