Sentence examples for normalization samples from inspiring English sources
After normalization, samples of the same type were combined and the mean expression value was used for subsequent analysis (see below).
Following data normalization, samples were ordered according to tissue type and the dataset was saved as an '.expression' file and then loaded into the network analysis tool BioLayout Express3D [ 30], as described previously [ 31].
After normalization, samples were examined using hierarchical clustering and pair-wise scatter plots to identify samples that severely deviated from other samples within the same strain (such samples were eliminated from further analysis).
After purification of PCR fragments and library normalization, sample pooling was performed by mixing equal volumes of each normalized DNA library, and the barcoded multiplexed library sequencing was performed on an Illumina MiSeq (Illumina).
This processing may involve background correction, normalization, sample selection, or feature/gene selection.
DSN cDNA normalization was carried out according to modified Illumina "DSN normalization sample prep application note".
DSN rRNA depletion is carried out following Illumina's "DSN normalization sample prep application note".
DSN cDNA normalization was carried out according to the modified Illumina "DSN normalization sample prep application note".
The total RNA libraries went through DSN treatment and PCR enrichment according to Illumina DSN Normalization Sample Preparation Guide (http://supportres.illumina.com/documents/myillumina/7836bd3e-3358-4834-b2f7-80f80acb4e3f/dsn_normalization_sampleprep_application_note_15014673_c.pdf).
Procedure controls included an input condition, obtained before DNA-protein complex sonication and further used during ChIP-Seq assays as normalization sample, and non-immunoprecipitated DNA (non-IP DNA), which was obtained just prior to Pax8 immunoprecipitation.
PCR amplification of the housekeeping gene β-actin was performed for each sample for normalization between samples.
