Sentence examples for normalization protocol from inspiring English sources
Differential expression between D. miranda males and females was calculated for both species using the log2 fold change on the normalized expression counts using the normalization protocol implemented in the R package DESeq (Anders and Huber, 2010) version 1.10.1.
We used these exogenous transcripts to normalize the endogenous transcripts in a two-step normalization protocol.
Although this may be an artifact of sequencing depth (i.e. low-abundance genes of interest may be present in only one of the two cDNA samples simply due to sampling effects rather than the normalization protocol per se), our data suggest that the normalized cDNA sample did not contribute disproportionately to gene discovery.
VBM was implemented in the VBM8 toolbox (http://dbm.neuro.uni-jena.de/vbm.html) with default parameters incorporating the DARTEL toolbox, which was used to obtain a high-dimensional normalization protocol [18].
We considered whether this was due to the normalization protocol and subsequent segmentation analysis.
The resulting, newly formed ds DNAs were then eliminated, while the sequences that remained single stranded (ss) were further used in the normalization protocol.
As with any quantitative technology, there is a fundamental need for quality assurance and normalization protocols.
However, good normalization protocols still have to be implemented to facilitate data exchange and comparison.
The observation that two very different normalization protocols yielded essentially identical results probably indicates that there is very little variance, either analytical or biological, among our samples.
Briefly, data normalization was performed using MetaboloAnalyst's built-in normalization protocols [ 65] including row-wise and column-wise normalization and auto-scaling procedures.
The studies, published until now, are a solid basis for discovery and establishing new biomarkers in PCa, but for further studies better, widely accepted, standardization, and normalization protocols are needed.
