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Following strict quality assessment procedures in both techniques, (RNA quality, cross-hybridisation of microarray probes, microarray spot intensity, qPCR primer design, and PCR efficiency) and data filtering after normalization (cut-offs for fold-changes and low microarray spot intensity) resulted in high correlation between qPCR and microarray data (Additional file 9) [ 60, 61].
Cut off.
Their legs cut off.
Cut off the apron.
Hands are cut off.
Their arms cut off.
Cut off the telephone.
You get cut off.
I was cut off.
"Cut off my feet.
Their head cut off.
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