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Equivalent analyses after a normalising data transformation will be carried out if non-normality of outcomes is evinced.
Normalising data can, however, reduce differences between individual patients by making them conform to a template and hence reduce the sensitivity to abnormalities.
They were analysed for side effects correlating Chemotherapy Toxicity Criteria with vitamin D levels, normalising data for general markers of patient health including C-reactive protein and albumin.
(Note that the lead role of higher areas was confirmed even after normalising data for the number of CA cells per area, removing the possibility that the observed phenomena be simply a result of scaling effects).
Expression of 6 selected miRNAs, including let-7b, let-7g, miR-1, miR-146, miR-16, and miR-17-5p, was validated using TaqMan MicroRNA Assays according to the manufacturer's instructions (Applied Biosystems, Foster City, CA, USA) and normalising data to U6 small nuclear RNA (RNU6; Applied Biosystems) expression.
Fold change in ABC transporter expression was determined using the comparative ct method, normalising data to peptidylprolyl isomerase A (PPIA; Lastowska et al, 2007).
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Interestingly, when normalising fate-mapping data to the initial contribution of PSU-derived cells to the wound epidermis, different PSU stem cell populations contribute in a comparable manner to long-term tissue maintenance (Page et al., 2013).
In the future we are looking to extend this package with additional methods for normalising and processing the data, and expand the number of formats from which interaction data can be imported.
The five steps in the CGH process were: data cleaning; normalising, cut-off, validation, and analysis.
Common normalising transformations of the data, such as the reciprocal, square root and natural log were unsuccessful and binary variables were therefore created from these continuous variables.
Normalisation of array CGH data has generally involved the use of methods originally developed for normalising gene expression microarray data (for a review, see [51]).
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