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To normalise values of the real-time PCR, recombinant miR-16 was used as a control.
Nevertheless the inclusion of multiple endogenous controls in our reactions would normalise values for such an overall reduction and thus would not affect the algorithm.
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Negative CH (normalised) values show that in addition to the CH released by the OPC, a part of the hydrated lime powder added externally, has also been consumed by the pozzolanic reaction.
To investigate the relation between B z and dH when they are far from zero, we normalise the values of a ij shown in Fig. 3a.
In all transfections, a β-galactosidase expression plasmid (300 ng) was used to normalise luciferase values.
Concentration data has been normalised to values obtained for the original (input) oil (see Table 1).
where the element symbols represent concentrations in the concretion normalised to values for a standard reference sample.
The graph shows normalised median values of fluorescence intensity measured using flow cytometry (mean of three independent experiments ± SEM for each concentration).
Quantification was assessed by the comparative Δ CT method normalising CT values to β-actin.
Quantification was performed by the comparative Δ CT method normalising CT values to β-actin.
Gene expression was quantified by the comparative cycle threshold (CT) method, normalising CT values to the housekeeping gene GAPDH and calculating relative expression values [ 23].
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