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The beta-values were converted to logit transformed M values, and quantile normalisation was used to normalise signal intensities to reduce inter-array variation [ 33].
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The data were normalised using the ExonRMA16 pre-processor and normalised signal values for individual probe-sets were standardised to the median value for the probe-set.
Briefly, single nucleotide polymorphisms were genotyped using the Human660W-Quad BeadChip (Illumina, San Diego, California, USA) and BeadStudio was used to call genotypes, normalise the signal intensity data and determine the log R ratio and B allele frequency at each single nucleotide polymorphism according to the standard Illumina protocols.
Four human genes were included in order to standardise the amount of applied cDNA and to normalise the signals between samples and references.
The microarray dataset contains two files (processed data, in.xlxs format): a file containing all miRNAs from miRBase version 17 and the associated RMA normalised intensity signal for all samples.
In order to visualize the density of aligned reads we produced so-called Hilbert-curves [ 14] of the distributions on the entire chromosome 1 for the IgG, MAX and IgG normalised MAX signal and from this it is clear that the IgG-signal is widely distributed over the whole chromosome whilst the MAX-signal seem to be more concentrated.
Log2 normalised averaged signals are provided for each probe.
Data were analysed by first subtracting the background and then normalising the signals using a LOWESS filter (Locally weighted Regression) (Bolstad et al, 2003).
The data were analysed by first subtracting the background and then normalising the signals using a LOWESS filter (locally weighted regression).
To normalise the luminescent signal to the number of cells present, a second plate was set up simultaneously and cells per well counted using a Beckman Coulter Z1 Coulter Counter (Beckman Coulter, Brea, CA, USA).
All measured values were normalised to the signal obtained from mCherry, which is tagged to the Peredox protein.
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