Sentence examples for normalisation samples from inspiring English sources

After normalisation, samples repeated across different plates showed good reproducibility (Additional file 1: Figure S3).

Following QC and normalisation, samples which were repeated across different plates showed good reproducibility (Additional file 1: Figure S3).

Samples from all subject groups and all brain regions were included in every batch together with standard normalisation samples.

For normalisation, sample and diluent volumes are automatically calculated and produced to obtain equal DNA concentrations for all samples (25 50 ng/μl).

We chose a sample from the centre of the distribution of the wild-type samples as the normalisation sample for the construction of the difference plots to standardise the interpretation.

The GAPDH RT PCR products were used as controls of sample loading for normalisation between samples.

To allow for normalisation of sample-to-sample variation, 25 fmol of synthetic C. elegans miRNA cel-miR-39 (5 μl of 5 nℳ miRNA mimic) (Qiagen, Hilden, Germany) was added to the denatured samples.

Normalisation between samples was carried out using a mouse monoclonal against GAPDH (ab9484, abcam) and the same secondary reagent on the lower part of the same blot.

Actin was detected for normalisation between samples using anti-beta-actin primary antibodies at the dilution of 1 5000 (Sigma-Aldrich, Saint Quentin Fallavier, France).

After normalisation the samples were hierarchically clustered according to their global expression profiles, to verify the biological accuracy of the data.

Double staining was performed to quantify actin expression for the normalisation between samples using anti-beta-actin primary antibodies (Sigma-Aldrich, Saint Quentin Fallavier, France) and Cy3 secondary antibodies (Jackson ImmunoResearch Laboratories, Interchim, Clichy, France).