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A within-array loess normalisation of intensities was applied.
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Relative gene expression levels were calculated by normalisation of band intensities to the reference gene.
Statistical analysis of protein abundance was performed on the value generated from normalisation of band intensity to the loading control.
Normalisation of background intensity was estimated from a set of built-in negative controls and subtracted from each methylation data point as performed in other studies [ 23, 24].
After background subtraction, photobleaching correction and normalisation of fluorescence intensity, the half-life of signal dissipation was determined by fitting a single exponential curve to the fluorescence intensity data over time.
The ratio-splitting operation includes error modelling of the channels of each ratio scan, group normalisation, forward transformation of intensities, group detrending, inter-slide error correction, and finally inverse transformation of intensities.
Our hypothesis is that normalisation of increased signal intensities on fluid-sensitive sequences on MRI is not required for a successful RTP and that low-signal intensity suggestive of fibrous tissues may be observed in the majority of clinically recovered hamstring injuries at RTP.
Eighty-nine per cent of the injuries showed intramuscular increased signal intensity on fluid-sensitive sequences on MRI at RTP. Normalisation of this increased signal intensity on MRI does not seem required for a successful RTP.
Normalisation of this increased signal intensity seems not required for a successful RTP.
Normalisation of the increased signal intensity (oedema) on MRI is not required for a successful return to play (RTP).
The findings of present article support clinicians with the interpretation of follow-up MRI after acute hamstring injury: Normalisation of the increased signal intensity (oedema) on MRI is not required for a successful return to play (RTP).
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