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For per gene normalisation, comparisons were conducted for the three expression values obtained across three biological replicates.
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Non-treatment controls were performed for normalisation and comparison.
VEGF (262 p M) was used as a positive control, whereas non-treatment controls provided normalisation and comparison.
EGM-2 was used as a positive control, while non-treatment controls were performed for normalisation and comparison.
VEGF (262 p M) was used as a positive control, whereas non-treatment controls were performed for normalisation and comparison.
EGM-2 was employed as a positive control, whereas non-treatment controls were performed for normalisation and comparison.
Non-treatment controls were performed for normalisation and comparison of EGCG and EA effects on in vitro angiogenesis.
Our high, even coverage enabled the detection of two multi-exonic duplications and three single or multi-exon deletions by cross-sample normalisation and comparison.
This sort of normalisation allows comparison of all arrays without biases when a majority of the spots on the arrays were giving positive hybridisation signals [ 12].
In order to validate comparisons, normalisation of frequencies and effect size significance measures were used.
Since the distributions defied further standard methods of normalisation to allow comparisons (square root, natural log and reciprocal), regressions, ANOVAs and t-tests were ruled out.
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