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If both L-strand DNA synthesis events terminated at the WANCY region, the normal termination site, the fragment between the two OL-like sequences would be synthesized twice.
This approach identified a new isoform of LDHB that arises from the readthrough of the normal termination codon resulting in translation of a C-terminally extended protein product harboring the peroxisomal targeting sequence.
However, suppression of normal termination codons could affect studies of mutant proteins containing for example fluorescent amino acids in living cells (in this case leading to a high background).
While these mitotic kinases can be inhibited by DNA damage checkpoint kinases, the mechanisms that promote their activation in response to normal termination of DNA replication are not well characterized.
These include leaky scanning of 40S ribosomal subunits past the most 5' AUG [2], the de novo recruitment of ribosomes to intercistronic internal ribosomal entry sites (IRESs; [3]); programmed ribosomal frameshifting [4], [5] and the circumvention of normal termination by programmed stop codon readthrough [6], [7].
(1) Normal Termination of Inflammation and Immune Reactions Could Be Associated with the M3 Phenotype of Macrophages.
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Human fetal liver tissue (15 wk gestation) was obtained from Advanced Bioscience Resources (Alameda, CA) following normal terminations and in accordance with nationally (UK and USA) approved ethical and legal guidelines.
And, who knows, maybe my whining prompted a quicker-than-normal termination of services?
The most direct evidence is that a ribosome toeprint generated at a normal translation termination codon is distinct from the toeprint of a ribosome terminating at a premature termination codon in a manner dependent on Upf1p (Amrani et al. 2004).
Readthrough transcripts arise when transcription initiates in one gene, continues past the normal transcription termination signals for that gene, and terminates within or at the end of a downstream gene on the same strand.
Then, we developed an engineered stable cell line using a tetracycline-controlled inducible lentiviral system for the conditional expression of mutant eRF1, which can minimize the potential effect on normal translation termination.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com