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Exact(28)
The truncated form of OsCIPK3/OsCK1 (without the C-terminal) has normal substrate phosphorylation activity but decreased autophosphorylation activity [82].
Cilastatin binds as a normal substrate and is orientated in a perfect near-attack conformer for formation of a tetrahedral intermediate with the zinc-bound water/hydroxide.
In addition, the incorporated abases competitively inhibit DNA polymerase α in opposition to the normal substrate deoxycytidine 5'-triphosphate [53].
The current findings may also have physiologic relevance to other situations such as the ischemic heart, which loses its normal substrate flexibility and becomes more dependent upon glucose as a metabolic fuel [28].
Is MANT-ATP a normal substrate for ATP hydrolysis?
For example, IBR5C129S may bind and sequester its substrate(s), thereby dampening normal substrate activity.
Similar(32)
Deoxynucleoside triphosphates (dNTPs) are the normal substrates for DNA synthesis catalyzed by polymerases such as HIV-1 reverse transcriptase (RT).
Clearly, insertions into the predicted sugar-binding domain of LevQ alleviated the requirement for normal substrates to be present for activation of the complex.
This binding would occur in competition with normal substrates.
Nevertheless, it is devoid of activity with normal substrates for lipoxygenases.
However, the role of catalytic site cooperativity was not extended to the catalytic function with normal substrates.
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