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Each cancellation group was first assigned 5 cancerous spectra and 7 normal spectra and the remaining spectra entered into the fifth group.
The correlation coefficients of each spectrum labeled as "C" by expert and the spectrum labeled as "T" by expert, noted as RCT, are calculated to investigate the difference of normal spectra and tumor spectra and its variation.
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So moving away from what we would consider a normal spectrum and into the UV range can dramatically alter the appearance of fossils revealing new details, and, on occasion, reveal something that's frankly beautiful.
The second major cluster contained all cancer spectra as well as one normal spectrum and another that is an obvious outlier within this group.
Two different multivariate analysis approaches were used to determine and visualize the pattern of similarities within and between cancer and normal spectra.
For this purpose, two thirds of the spectra, comprised of a representative proportion of tumour and normal spectra, were randomly assigned to a training dataset, with the remaining third being used as an independent test dataset.
The prominent absorbance bands observed in both cancer and normal spectra are characteristic of vibration modes assumed to represent functional groups in cellular molecules including proteins, carbohydrates and nucleic acids [ 26, 27, 24].
An alternative 2D method that correlates pure shift and normal spectra is that of Giraud et al.,[ 44] which is less sensitive but has the significant advantage of allowing phase sensitive display, and has been applied successfully to the analysis of spectra of samples in aligned media.
Collagen assignment was again higher in both "true" and "adjacent to disease" normal spectra whereas DNA was higher in low and high grade dysplasia spectra [ 32].
Each subset consisted of 26 cancerous and 36 normal spectra from different patients.
The principal qualitative differences between "true" normal and "previous disease" normal spectra were found in the 1200 1400 cm−1 range where assignments to proteins and collagen type I were higher in "true" normal spectra whilst the DNA and glycogen assignments (~1330 cm−1) were higher in "previous disease" normal Raman spectra [ 32].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com