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Oocytes were classified as morphologically normal oocytes (MNO) and deemed suitable for incubation if ≥3 compact layers of cumulus cells and a homogeneous, evenly granulated cytoplasm were observed.
Considering the possible deviation derived from mechanical stress during chromosome spreading, and the possibility that even normal oocytes may have more or less than 20 chromosomes, we conducted chromosome spreading with exact chromosome numbers not only in oocyte but also in polar body.
No significant differences were observed in the proportion of normal oocytes between the two groups.
Whenever possible, only chromosomally normal oocytes were inseminated and up to a maximum of three per patient as established by the national law on IVF (Benagiano and Gianaroli, 2004), whereas the remaining chromosomally normal oocytes were cryopreserved.
After incubation, morphologically normal oocytes were selected, and fertilization was initially assessed by observing pronuclei formation at this stage.
Interestingly, we observed centrosome (p-value = 3.32 × 10-5) but not spindle related terms in pink module (normal oocytes).
Similar(39)
The extent that harnessing normal oocyte polarity would be critical in the engineering of eggs from stem cells for reproductive regenerative medicine is also discussed.
To examine whether similar levels of phospholipase C zeta (PLC-ζ) protein are present in sperm from men whose ejaculates resulted in normal oocyte activation, and to examine whether a predominant pattern of PLC-ζ localization is linked to normal oocyte activation ability.
Transcripts from fancd1(brca2) localize at the animal pole of the zebrafish oocyte cytoplasm and are required for normal oocyte nuclear architecture, for normal embryonic development, and for preventing ovarian tumors.
It is concluded that SHB regulates normal oocyte and follicle development and that perturbation of SHB signaling causes defective meiosis I and early embryo development.
This posterior Orb-positive cell often resembled a normal oocyte in size, shape and nuclear morphology; the former characteristics being most obvious in late stage follicles (Fig. 2C, D).
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