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The treatment would require radiation and chemotherapy at levels that would destory all of her remaining normal marrow cells, bringing her close to death and leaving her with no immune defenses whatsoever.
To determine the efficacy of pharmacological purging by ATP, we designed a murine system to mimic the conditions expected in the clinical setting of autologous transplantation using simulated partial remission marrow (SPRM) which was prepared by mixing normal marrow cells and L1210 cells at a ratio of 9 1.
Note normal marrow signal intensity (M).
Note normal marrow signal in the first metatarsal head (asterisks).
An infiltration with less than 20%% neoplastic cells cannot be distinguished from normal marrow with standard MR pulse sequences [24].
Both benign and malignant tumours usually have inferior signal intensity to the normal marrow signal in T1WI [13].
At 3 months postgrafting, the proportion of patients positive to DNCB was equal to that of normal marrow donors, but thereafter it was lower until 2 years.
The proportion of patients positive to recall antigens was lower than that of normal marrow donors until 4 years after grafting.
Transplantation of normal marrow to a lethally irradiated hemophilic dog did not result in an increase in factor VIII during 34 days of observation.
VP-16 was selectivity toxic to the parental leukemic cells, with IC-50 values of 2 microM for CEM cells, 1.5 microM for K562 cells, and 12 microM for normal marrow CFU-GM.
Cyclosporine, 6 microMs, and verapamil, 20 microM, alone were nontoxic to bone marrow CFU-GM, and did not significantly increase the toxicity of VP-16 to normal marrow cells or to the two drug-sensitive leukemic cell lines.
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