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The cytotoxicity assay was carried out against normal lymphocytes.
Simultaneously the drug does not remarkably effect the normal lymphocytes (Additional file 1: Figure S5).
The normal lymphocytes were found to be non-toxic at this dose.
siRNA-mix and Glivec did not affect significantly the viability of normal lymphocytes.
Thirty-nine (25%) patients had recovered a normal lymphocytes count within the first 2 days in the ICU.
However, the stiffness of normal lymphocytes is 2.28 ± 0.49 mN/m, and it is 4.32 ± 0.3 mN/m for Jurkat lymphocytes.
Normal lymphocytes (1 × 106) were also seeded on 96-well and treated with IC50 dose of cell lines with respect to standard drug imatinib mesylate (10 μg/ml).
DNA from matching normal lymphocytes for samples from Southampton were extracted as described previously [33].
In contrast, normal lymphocytes and H460 cell line had diploid copies of the CSNK2A1P gene (Fig. 1C and D).
In addition to genome differences, microarrays of normal lymphocytes from randomly selected subjects reveal effects due to time of collection, age, sex and nutrition [13].
On the contrary, we recorded a 30% of cases in which VAV1 mRNA levels were at least 2 fold higher than those found in normal lymphocytes.
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