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Normal Eve expression is manifested by seven precisely positioned stripes along the antero-posterior axis of the embryo [37].
In contrast, all Ppt1 mutant strains outcrossed to wild type Oregon-R, Df(1 446-20/+, Ppt1A179T/+, and Ppt1S77F/+, all showed normal EVE expression (Tables 1 and 2).
Additionally, dcr-2R416x embryos displayed normal Eve expression when developing at alternating uniform temperatures, where the temperature was alternated between 20°C and 27°C every 15 or every 30 minutes (data not shown).
To further show that abnormal Bcd can lead to normal Eve patterning, we determined the intensity profile of Bcd and Eve in immunostained wild-type embryos that were placed in a microfluidic device exposed to a temperature step approximately 20 minutes after fertilization.
We found that dcr-2R416x (Figure 2A) and dcr-2L811fsX (Figure 2C) embryos displayed normal Eve expression patterns at a uniform temperature of 24°C, as well as at uniform temperatures of 20°C and 27°C (Figure S1A, C D, F).
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