Sentence examples for nonstop degradation from inspiring English sources
According to "Who Is Sonny Rollins," a short BBC documentary from 1968, Rollins — who had been addicted to heroin in the late nineteen-forties and early fifties but sweated it out at the Lexington Narcotics Farm, a combination federal prison and rehabilitation facility, in Lexington, Kentucky — was exhausted by what he understood as a culture of nonstop degradation.
Nonstop decay results in posttranscriptional degradation of transcripts without an in-frame stop codon prior to the polyadenylation signal and is conserved throughout eukaryotes (Gatfield et al. 2003).
More recently, the Rkr1/ ligase3 ligase was found to associate with ribosomes and to target nascent nonstop polypeptides that are stalled during translation for degradation (Bengtson and Joazeiro 2010).
The smeary bands observed in lane 4 most likely indicate that the position of 5′ and/or 3′ ends of 3′ extensions is not uniform, which is probably due to degradation and/or variable poly(A) tail and nonstop PCR amplification.
In the absence of Ski7 or the exosome, nonstop mRNAs are subject to accelerated decapping and 5′ to 3′ degradation (Inada and Aiba 2005), perhaps because of the failure to recycle terminating ribosomes for continued efficient translation initiation.
Although some RNA degradation pathways can apparently act on polyadenylated transcripts, e.g. nonstop decay (NSD), nonsense-mediated decay (NMD), and endonucleolytic cleavage (RNAi), the majority of 5' to 3' and 3' to 5' exonucleolytic activities require the prior removal of the poly-A tail.
