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None was sufficient.
Unfortunately, non-specific precipitation of Gli proteins was detected in cell extracts incubated with Protein G beads alone and although various blocking and extensive washing conditions were tested, none was sufficient to reliably eliminate the non-specific precipitation.
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But they say none is sufficient to account for the precipitous price jumps in cities like Chicago and Milwaukee.
While each of these speculations has scientific support, none are sufficient to explain an illness that afflicts so many people.
The relationships among usability, utility, and adoptability are complicated: although they can be mutually reinforcing, none is sufficient (nor perhaps even necessary) to achieve the other two.
Herein, a number of key molecules whose expression levels are interrelated, including both EGFR and uPAR, are required but none are sufficient in the absence of other keys molecules in promoting tumor progression.
None are sufficient, however, to invalidate the data and results of Seralini et al. Most critics comment on the number of deaths in control and experimental groups, for example pointing out that male rats that were given Roundup at the highest concentration actually lived a little longer than the controls.
While all were valuable, none were sufficient.
It is apparent that each of the three approaches is necessary but none is sufficient on its own but synergistic connections are crucial for making an impact.
It is now recognized that the practice of medicine is influenced by many factors other than evidence, although none is sufficient alone to explain clinical decision-making [ 16, 29, 30].
Existing methodological approaches go some way towards enabling a thorough understanding of the mental health needs of such hard-to-reach groups, and to generating new solutions to those needs: but none is sufficient.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com