Sentence examples similar to non-stringent screening from inspiring English sources

A 1.5 kb cDNA fragment amplified from the 3' part of Selaginella RpoT [ 26] was used as a P-labelled hybridization probe to screen the Nuphar BAC library, containing 165,888 independent clones on nine individual filters, under non-stringent conditions (58°C).

We also performed these same analyses for each fusion primer set separately using stringent and non-stringent dimer classification and found similar results (data not shown).

Low bioavailability was also indicated by low amounts of NPs recovered during extraction with non-stringent extractants.

P. aeruginosa occupies a diversity of ecological niches, last but not least due the versatile and non-stringent metabolic requirements.

A non-stringent expect value (E-value) of < e-10 was chosen as the threshold.

GeneChips were washed with a series of non-stringent (25°C) and stringent (50°C) solutions containing variable amounts of MES, Tween20 and SSPE.

After hybridisation, the arrays were washed in a GeneChip Fluidics Station 400 with a non-stringent wash buffer at 25°C followed by a stringent wash buffer at 50°C.

GeneChips were washed with a series of non-stringent (25°C) and stringent (50°C) solutions containing variable amounts of MES buffer, tween 20 and SSPE buffer.

Post-hybridization processing included a stringent wash to remove unbound and non-specifically hybridized target molecules, a staining step with a Cy™5-Streptavidin conjugate, and several non-stringent washing steps to remove unbound conjugate.

The TRACKER program computes pairwise local alignments of all input sequences using BLASTZ [ 42] with non-stringent settings.

The mean of negative controls is a non-stringent correction that maximizes sensitivity in exchange for increased false positives.