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We also performed these same analyses for each fusion primer set separately using stringent and non-stringent dimer classification and found similar results (data not shown).
Low bioavailability was also indicated by low amounts of NPs recovered during extraction with non-stringent extractants.
However, unlike AMO NIL MMS4, Delo-Katiobond has a purely organic chemistry so it may be etched in an oxygen plasma, thus enabling non-stringent residual layer removal [15].
For example, while there is a very stringent moral presumption against killing an unimpaired adult human being, there might be only strong but non-stringent reasons not to kill a dog, and very weak reasons not to kill a fish.
P. aeruginosa occupies a diversity of ecological niches, last but not least due the versatile and non-stringent metabolic requirements.
A non-stringent expect value (E-value) of < e-10 was chosen as the threshold.
GeneChips were washed with a series of non-stringent (25°C) and stringent (50°C) solutions containing variable amounts of MES, Tween20 and SSPE.
After hybridisation, the arrays were washed in a GeneChip Fluidics Station 400 with a non-stringent wash buffer at 25°C followed by a stringent wash buffer at 50°C.
GeneChips were washed with a series of non-stringent (25°C) and stringent (50°C) solutions containing variable amounts of MES buffer, tween 20 and SSPE buffer.
Post-hybridization processing included a stringent wash to remove unbound and non-specifically hybridized target molecules, a staining step with a Cy™5-Streptavidin conjugate, and several non-stringent washing steps to remove unbound conjugate.
The TRACKER program computes pairwise local alignments of all input sequences using BLASTZ [ 42] with non-stringent settings.
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