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Exact(34)

The kinetic parameters of variants AbOPHI211A, AbOPHL156M and AbOPHH268L were determined by non-linear fitting.

fB is then adapted to penalized non-linear fitting including Poisson maximum likelihood estimation by MC simulations.

Apparent KD values were then calculated from the plots ΔA = f [Arb]), by non-linear fitting.

For the FRAP analysis, raw data were background subtracted, corrected for acquisition-induced bleaching and normalized, and the curve was fitted from pooled values with Prism5 software using one-phase association non-linear fitting.

Computations were performed using STATISTICA 6.0, and isotope incorporation data were fitted using a Marquardt non-linear fitting routine (NLIN, SAS, Cary, NC, USA).

Isotope turnover rates were quantified by fitting the data using a Marquardt non-linear fitting routine (NLIN, SAS) using the following equations: where y is δX (C or N), a is the isotopic value approached asymptotically (δX, b is the total change in isotopic values after the diets were switched (δX−δX t)), c is the turnover rate, and t is the time in days since the switch.

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Similar(26)

(b) Non-linear fits to Eq. (3) which were used to determine whether, or not, the inhibition was cooperative and, if so, the value of the Hill coefficient (h ) – see Section 2.3.

The fitting procedure was implemented in Origin® software non-linear fitter.

Solid lines are the non-linear fits of the data using GraphPad Prism (version 4.00, San Diego, CA, USA).

The kinetic growth parameters were evaluated through a non-linear fit.

A non-linear fit between the rain rate and the SI is established for rain measurement.

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