We show that this substitution has no effect on Shroom3 expression or localization but ablates Rock binding and renders Shroom3 non-functional for the ability to regulate cell morphology.
These data indicate that all disease-linked truncating mutations localised upstream of the NLS are likely to be non-functional, losing their ability to translocate to the nucleus [ 9].
Generally, pancreatic neuroendocrine tumors are classified as either functional or non-functional depending on their ability to secrete biologically active hormones.
They were previously referred to as 'junk DNA' or 'dead genes' because they were thought to be completely non-functional, lacking even the ability to encode RNA.
Further, the mutant protein was completely non-functional in terms of its ability to transactivate a series of p53-responsive genes (p21 WAF1, bax, PIG3), to transrepress a target gene and to inhibit colony growth in transfected Saos-2 cells.
Roots were considered non-functional teeth with respect to chewing ability, and therefore considered as missing teeth.
We believe that the ability to distinguish functional from non-functional interactions within living cells is an important research area and will only increase in importance in the future.
To assess SuRFR's ability to distinguish functional variants from non-functional, a control set of non-functional variants was required.
The ability to identify truncation mutations in five of the seven SBE targets and putative non-functional amino acid substitutions in the remaining genes highlights the power of this approach for functional gene analysis.
In 1933, when FDR took power, global banking was essentially non-functional.
Thus, it is possible that this ligand might generate a non-functional GR/TIF2 complex or that its antagonistic effect would reside on its ability to modulate the dynamics of GR-DNA interaction as previously suggested [60] and confirmed here.
