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The major obstacle has traditionally been poor or non-existent reference genomes combined with the high cost of developing oligo capture arrays required for exome sequencing, the most popular method for genotyping in humans.
In an organism with a contig-level or non-existent reference genome, selecting an enzyme with a sparse profile so each marker is covered in a large number of samples may be desirable.
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"He is a non-existent character.
In the case of datasets from organisms with non-existent or incomplete reference genomes, namely ones that exist as unscaffolded contigs, algorithms designed for humans fail entirely.
She sends a series of prospective tenants - a couple whose credit references are non-existent & who had stolen all the furniture from the house they rented previously; a counterfeiter; two gangsters who deal only in cash, & anybody that doesn't like it "... they're liable to wind up with their feet in a barrel of cement".
Applying the so-called "nonsense formats", designed against non-existent components, to the NIBSC reference preparation of rheumatoid factor (RF), no response was found with the new assay, whereas a clear RF dose-dependent interfering signal was observed with the original assay designed for tumor tissue extracts.
(For more on reference to non-existent objects, see the entry on nonexistent objects).
Correcting inconsistencies, such as references to articles on implementing acts in the delegation of power, references to non-existent provisions, etc. as well as changing indeterminate period of delegation of power to five years, tacitly extended for identical periods of time.
In addition, each miRNA target site was cross referenced with miRBase [ 24] and targets referencing dead or non-existent miRNAs were excluded.
McCain's reference to a non-existent "Iraq-Pakistan border" should at least be a flashing yellow light for those who expect a U.S. president to have a grasp of foreign policy nuances.
Any reference to earlier architecture is practically non-existent yet the Atomium has since become a symbol of a future that never was.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com