Accordingly, DEP has been used to distinguish between live, dead, and non-viable cells[23], [24], [25], as well as between different cells types[26].
Limiting the use of these samples to 10% dead cells in each individual subset, 13 experiments were used with mean contents of 3.7±2.8% and 6.8±4% non-viable CD25− and CD25+ cells, respectively.
Injection of non-viable spores resulted in less neutrophil recruitment than injection with viable spores after low- and high-dose challenge (LD Mc viable versus non-viable P<0.05; HD Mc viable versus non-viable not significant; Fig. 5E and Table S1B).
14 Prevalence of above-cut-off PHQ-9 scores was similar among women with non-viable (4/21; 19%) and viable (6/33; 18%) pregnancy outcomes.
In addition, Lm-infected p47phox−/− spleens contained significantly more (P<0.001) non-viable CD4+ and CD8+ T lymphocytes than similarly infected WT spleens on post infection day 3.
Significantly lower numbers of neutrophils at the injection site were detected in larvae injected with non-viable spores compared with larvae injected with viable spores (LD Mc versus HD Mc non-viable P<0.01; HD Mc viable versus HD Mc non-viable P<0.01; Fig. 2B and Table S1B).
Injection of non-viable spores showed less neutrophil recruitment than injection with viable spores after low- and high-dose challenge (LD Mc viable versus non-viable not significant; HD Mc viable versus non-viable P<0.001; Fig. 5E and Table S1B).
Macrophage recruitment after high-dose challenge with viable or non-viable spores was also significantly higher than after low-dose challenge with viable or non-viable spores, respectively (LD Mc versus HD Mc viable P<0.001; LD Mc versus HD Mc non-viable P<0.05).
The proportion of PKH2+PI+ (non-viable) K562 cells at each effector to target cell ratio will be determined after 15 min by flow cytometry.
After fertilization by a normal male gamete, these two polar nuclei should normally generate a non-viable 5 x endosperm with a 4 1 ratio.
Using trypan blue exclusion assay, the proportion of non-viable LEF2 cells was moderately reduced in the presence of ketone bodies whereas MTT absorbance remained relatively constant.
