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Non specific staining by the Cy5 conjugated anti mouse antibodies is shown in the IgG control.
Thus the patients were divided into non-staining and staining groups.
Five-year survival of non-staining and staining groups were 39.4% and 10.7% (p < 0.00005); five-year recurrence-free survival of these groups were 29.8% and ≤ 7.5% (p < 0.00005), respectively.
Watercolor paints may also be either non-staining or staining.
Chromogenic signals were evaluated by brightfield microscopy and semi-quantified with regard to percentage of cells stained (0 100%) and the staining intensity (0: non-staining, 1+: weak staining, 2 +: moderate staining, and 3 +: strong staining).
Figure 1 shows representative sample cores of non-staining, weakly staining, intermediate and strongly staining cores.
Remember to use non-stain cooking flour.
Other extra-neural tissues contained a small number of non-specifically stained cells.
Plants that were hemizygous for MSP constructs showed 1 1 segregation of GUS staining and non-staining spores consistent with gametophytic expression (data not shown).
Also look for good cold working characteristics, resistance to acids/alkalis and water (especially water), and preferably non-staining (black grease stains are not attractive on your shirt and pants).
To control for non-specific staining, adjacent sections were stained as above, without the primary antibody.
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