The exact function of miR-18a remains unknown, however one study using lentiviral mediated antagomir delivery into K562 cells found a positive role for miR-18a in cellular proliferation [47], possibly explaining its absence in terminally differentiated non proliferating neutrophils.
It has been also found that the expression of the intracellular domain of DELTA-1 resulted in a non proliferating senescent-like cell phenotype [43].
When stationary (non proliferating) liquid populations of yeast wild-type cells grown on SCGlu were shifted to solid SCGal or SCGly, only 65% or 44%, respectively, of seeded cells were able to form a colony (Fig. 3A).
Immunohistochemical studies with a specific, in-house made anti-GPR17 antibody showed that, in the intact mouse telencephalon, GPR17 is present on both neurons and in a subpopulation of ramified non proliferating Olig2+ or NG2+ precursor cells dispersed in both gray and white matter.
Interestingly, as detected by the lack of Ki-67, plasma cells present in the salivary glands of all three groups tested were non proliferating cells.
This difference and the observation that Mad1 is highly expressed only in non proliferating, post-mitotic cells while Mxi1 is present in cycling cells (Hurlin et al, 1995), suggests that these two proteins affect cell cycle progression at different phases.
For Figure #3A, I understand well the two upper panels: the panel PBMC shows non proliferating PBMC (all the cells are brightly stained with CSFE) and the panel PBMC + Anti CD3/CD28 shows maximal proliferation with all the cells (gated for CD3) exhibiting decreased CSFE fluorescence due to CSFE dilution occurring during cell division.
In co-cultures of tumor cells together with irradiated non proliferating NPC we have in previous studies shown that HiB5 and ST14A can inhibit the tumor proliferation of N32 and N29 in vitro, but not of N25, thereby giving us a potential model system for measuring the antitumor effect seen in vivo.
And competitors, both nouveau and non, have proliferated, from Cirque du Soleil to New York's Big Apple Circus.
Subsequently, CD8+ T cells were stimulated with anti-CD3 and anti-CD28 antibodies to obtain non-specific proliferated T cells.
The specificity study showed that LD exerted a moderate cytotoxic action against non-proliferating and proliferating blood lymphocytes but not apoptosis.
