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Baseline levels were normalized using the 500 ms interval prior to the non-probe stimulus.
Baseline levels of PD were normalized using the 500 ms interval prior to the non-probe stimulus.
Non-probe stimuli were presented for 1000 ms followed by a fixation cross which varied in duration from 900 2300 milliseconds (mean duration 1500 ms).
Quality control of GE markers removed 21,198 non-RefSeq probes (11,622 probes from UniGene and 9,576 probes from Gnomon).
N ! where α = number of significant probes ∈ P. β = number of non significant probes∈ P. γ = number of significant probes ∉ P. δ = number of non significant probes ∉ P. S = number of significant probes in the array.
Non-specific probes and probes on the X and Y chromosomes were removed [ 62, 63].
Probes were then floored to a value of 20 to avoid superficially high ratios in respect to non expressed probes.
Removal of control and non-responsive probes identified 12,349 probe sets.
F = number of non significant probes in the array.
For completeness, the complete datasets (including also the non overlapping probes) were also analyzed in parallel.
After high – stringency washing of non – hybridized probes, the macroarrays were digitally evaluated.
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