Unsurprisingly, when Pol I-Alexa647 is used for internalization by electroporation, bright non-internalized spots are observed that correspond to protein aggregates.
Transcriptomic analysis using an oligoarray covering the whole S. aureus genome was performed at two post-internalization times and compared to gene expression of non-internalized bacteria.
Although electroporation allows high internalization efficiencies to be achieved, the amount of non-internalized fluorescent molecules that remain in cell suspension after electroporation is typically much higher than the amount of internalized molecules.
In contrast the low-internalizing agonist remains fully active following the second administration, demonstrating that non-internalized receptors remain functional.
The cells were then placed at 4°C to prevent bead internalization, stained with a human IgG-specific antibody (Jackson ImmunoResearch) to mark non-internalized beads and then fixed.
Arrowheads show the non-internalized anti-ICAM-1/NPs (red).
This terminology, unfortunately, is misleading as externalities are by definition non-internalized benefits.
The fraction of non-internalized, i.e. membrane-bound activity was 1% to 3% in all experiments (exemplarily shown for 177Lu-10 and 177Lu-11 in Figure 5).
This theory further suggests internalized motivation and non-internalized motivation influence people differently; people demonstrate a behavior to a greater extent when they perceive that the motivation to do it comes from themselves rather than from an external, controlling cause.
Furthermore, although there is more trastuzumab at the tumour at 72 h, the amount of non-internalized trastuzumab available for the reaction with [18F]TAF might in fact be the same.
Non-internalized motivation, on the other hand, works when people act mainly due to either the expectation to receive a reward (external motivation) or the desire to avoid feeling guilty (introjected motivation) Deci and Ryan (1991, 2000).
