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Induced plants were kept separately from non induced plants to avoid induction via ethanol vapor.
Total RNA and mRNA were isolated from chondrogenic induced pellet cultures, from non induced pellet cultures and from non-induced single layer cell cultures.
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NI: non-induced; I: induced; D: day.
Ratios of each two neighbouring wells (induced versus non-induced) were determined and a mean value (induced versus non-induced) was calculated for each microtitre plate.
Conversely, very few promoters (induced 0.1%%, non-induced 0.4 %) were located distal to non-induced DHSSs, while a substantial number of TSS clusters (induced 11%%, non-induced 13%%) were located distal to induced DHSSs.
(C ) TEM of non-induced (a b) and induced IFT27 RNAi cells (c d).
(B ) Growth curve of non-induced (blue) and induced (red) IFT27 RNAi cells.
(C ) Growth curves of the non-induced (blue) and induced (red) IFT27 RNAiRES cells.
A substantial number of clusters (induced TSS clusters 11%%, non-induced TSS clusters 14%%) were located proximal to the closest non-induced DHSSs, whereas only few TSS clusters (induced 1%%, non-induced 1 %) were located proximal to VPA-induced DHSSs.
By comparison, non-induced monocytes, which have minimal Sn expression (Fig. 2), exhibited low luciferase activity.
Blue indicates non-induced control; red indicates TbHydin RNAi-induced.
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