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Non incorporated cryptococcal cells were removed by carefully washing the macrophage cell layers three times with PBS.
cDNA were further purified by utilizing CentriCept gelfitration plates to remove non incorporated primer and dNTP.
Non incorporated nucleotides were removed using ProbeQuant G-50 microcolumns (Amersham Biosciences).
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"This negatively affects any spontaneous or innovative types of social economy organisations and non-incorporated entities," he says.
He also knocked a further percentage point off the top rate of income tax, to which all non-incorporated companies are liable.
The enterprises generating value added are therefore subdivided into incorporated and non-incorporated companies in order to account for differences in tax treatment.
These pooled data include results from approximately 140,000 financial statements per year of non-financial enterprises, including both incorporated and non-incorporated companies.
The systems have high visible light harvesting, high hydrogen evolution rate and long electron hole lifetimes compared with non-incorporated systems.
Furthermore, Census data indicate nearly a quarter of all 2007 revenue in the general freight trucking industry was made by businesses with no paid employees, i.e., mostly self-employed people operating non-incorporated businesses.
While each municipality operates as a separate and independent local government with executive, legislative and judicial power, county governments maintain jurisdiction over both incorporated cities and non-incorporated areas within their boundaries, but have executive power but no legislative or judicial power.
Non-incorporated free biarsenical dye was removed from the virions by sucrose density gradient centrifugation.
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