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Conserved elements were predicted from both referenced alignments using a phylogenetic hidden Markov model (HMM) consisting of two states (conserved and non-conserved) implemented in phastCons [ 15].

A difference score between levels of contributions in the implemented versus non-implemented punishment blocks was calculated (contributions in the implemented punishment condition minus contributions in the non-implemented punishment condition: a positive score indicates that more was given in the implemented condition than the non-implemented condition).

Individuals contributed more under the threat of punishment (both implemented and non-implemented).

Participants took part in a series of one-shot Public Goods Games (PGGs) facing two punishment conditions (implemented and non-implemented) and two standard non-punishment PGGs.

In addition, to ensure that the order of these three blocks (implemented punishment, non-implemented punishment and a standard game) did not have any effect on the contribution levels, the order of three blocks was randomized (see details below).

Punishment contingencies (either implemented or non-implemented) were fixed for all participants and did not depend either on other players' contribution levels or participants' individual contribution levels (unless they contributed more than 17 MUs on the "punishing" trial).

Individual differences in the punishment sensitivity have been linked to both threat responses (flight, fight, fear system, or the FFFS) and to the response to the uncertainty of punishment (BIS-anxiety).The paper, therefore, examines if individual differences in BIS-anxiety and FFFS can explain some of the variability in free riding in the face of implemented and non-implemented punishment.

The gunman invariably was 2 inches taller than a guy holding a non-threatening implement, even one that requires strength to use.

In-service training in HIV medicine was paid for and delivered by non-government implementing partners with accreditation provided by the Ministry of Health.

This model should also work in terms of perspective risks connected to implementing or non-implementing these interests.

Cities like Charlotte implemented non-discrimination laws based entirely on business case – knowing that such laws would make them attractive to out-of-state businesses and investors, explained Jonathan D Lovitz, vice-president of external affairs at the National Gay and Lesbian Chamber of Commerce.

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