False Discovery Rate (FDR) analysis separated Golden Retriever and non-Golden Retriever samples into distinct groups with a 5-gene signature of MHC DLA88, Forkhead box protein F1, Thrombospondin-3 precursor, zinc finger protein 322A, and NAD(P) dependent steroid dehydrogenase.
Naturally occurring hemangiosarcomas of Golden Retrievers clustered separately from those of non-Golden Retrievers, with contributions from transcription factors, survival factors, and from pro-inflammatory and angiogenic genes, and which were exclusively present in hemangiosarcoma and not in other tumors or normal cells (i.e., they were not due simply to variation in these genes among breeds).
Considering that Oklahoma City has been holding fairly steady with Russell Westbrook alone at the helm, this probably means real bad news for the non-Golden State portion of the Western Conference.
At the gate, my hard-won Delta GoldMegaElite status is reduced to nothing when the local T.S.A. subjects me to a full Ben Gurion Airpat-downde pat-down complete with simulated genital swipe, in full view of the non-Golden passengers.
After normalization and filtering, 13,758 genes remained for a comparison of Golden Retriever to non-Golden Retriever samples.
Thus, the expression of genes in the tumor was predictably modulated by the dog's Golden Retriever and non-Golden Retriever background.
We included each of the non-Golden Retrievers (Dal-4, Joey, and CHAD-P9) and three Golden Retrievers (CHAD G6, CHAD G4, and Frog) for this analysis.
Specifically, was this dog more similar to Golden Retrievers, to non-Golden Retrievers, or would it reflect a "mixture" of both?
Figure 3A shows immunocytochemical staining and immunoblotting for VEGFR1 and VEGFR2 in cell lines derived from Golden Retrievers and from non-Golden Retrievers.
Among genes whose expression differed between Golden Retrievers and non-Golden Retrievers, a disproportionately high number of genes encode transcription factors.
Another gene that was underexpressed in Golden Retrievers with hemangiosarcoma compared to non-Golden Retrievers is TSP-3, a member of the Thrombospondin family.
