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Probes were then floored to a value of 20 to avoid superficially high ratios in respect to non expressed probes.
We found that each gene was represented by a mixed set of expressed and non expressed probes.
Nectin-4, non expressed in normal cells, is prominently expressed in breast carcinoma cell lines and in breast tumor samples.
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Unigenes were called expressed, marginally expressed or non-expressed using the Agilent microarray.
As can be seen, however, the swm1Δ/wild-type expression ratio is generally high for non-expressed and weakly expressed genes, suggesting that (globally) Swm1 represses these non-expressed and weakly expressed genes.
Non-expressed (low mean intensities) and non-informative (low standard deviations) features were filtered.
At the expression level, a retrocopy can be either expressed or non-expressed.
Line TC-52, which initially contained pmi, non-expressed 1Dx5, and expressed 1Dy10 HMW glutenin subunit transgenes, was further investigated.
The remaining genes, both expressed and non-expressed, were used as test sets.
The threshold obtained (29.9 arbitrary units) was then used to classify MED4 probes as "expressed" or "non-expressed".
Patterns for histone density and FAIRE were consistent with open, nucleosome-poor and closed, nucleosome-rich chromatin states for expressed and non-expressed genes respectively (Supplementary Figure S5).
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