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After incubation, cells were washed twice with PBS and non-enzymatically detached as before in 500 µL cell dissociation solution.
Sub-confluent N2a cells in 100 mm Petri dishes were washed twice in PBS and non-enzymatically detached by incubating 5 min at 37°C in cell dissociation solution.
Cells were then non-enzymatically detached and subjected to FACS analyses as previously described [ 36].
The only surprises that Riley offers in "Listen to Me Marlon" are negative the detached, non-specific, heavy-handed banalization of the astonishing materials that he had the privilege to access.
An obvious problem is that claims involving that vocabulary cannot then be non-question-beggingly detached from the scope of that definition.
To determine the surface expression of CD20, CD133, CD44 and CD24 10 cells were detached non-enzymatically with 2.5 mM EDTA and incubated with the antibodies according to manufacturer's instructions in PBS containing 1% BSA for 1 h at 4°C.
In inhomogeneous solids, many non-dissolved grains are detached in typical conditions of temperature and activation energies, leading to ratios of detached and dissolved masses of order g, for g ≫ 1.
They were intensified by the Treaty of Sèvres (August 1920), which not only detached all non-Turkish regions from the empire but also gave parts of the Turkish homeland to Greece and other non-Muslim powers.
In keeping with his characters, Leconte's storytelling style, though, is detached and non-committed – one reason why the film never quite tugs at the emotions in the way that might have been expected.
The image was detached into non-overlapping 8×8 patches.
Target cells (5×105) were detached using non-enzymatic cell dissociation solution.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com