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Binding reactions were performed and analysed using 5% non-denaturing gels.
The DNA/RNA-protein complexes were analyzed by 6% non-denaturing PAGE.
Total protein extracts were separated by SDS-PAGE under denaturing and non-denaturing conditions (figure 1A).
A standard protocol was used for denaturing Southern blot hybridization [ 29], while for non-denaturing hybridization, DNA was transferred to membranes under non-denaturing conditions.
Sodium dodecyl sulfate (SDS) was omitted for non-denaturing gels.
Classification of SOD isoforms was performed by non-denaturing PAGE.
Catalase (EC 1.11.1.6) activity was observed after protein separation by non-denaturing electrophoresis.
The quality of dsRNA was assessed by non-denaturing agarose gel electrophoresis.
Fig. 5 SOD activity staining following non-denaturing PAGE analysis of P. ananatis CCT 7673 extracts.
SOD activity was assayed using non-denaturing PAGE gels, essentially as described (Gratão et al. 2015).
Amplification products were separated by electrophoresis in 3.5% agarose gel or 8.0% non-denaturing PAGE.
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