IL-1β then acts primarily on non bone-marrow-derived cells, such as capillary endothelial cells, by triggering MyD88-dependent events [ 5, 6].
In general, the tissue source for MSCs can be categorized as bone marrow or non-bone marrow.
First, we chose femoral bone marrow and adipose tissue because these are major sources of bone marrow-type and non-bone marrow MSCs, respectively.
However, BMP was named for the ability to form bone, and the name implies that they are capable of inducing the osteogenic differentiation of non-bone cells.
Matsumoto, M.et al. Distinct roles of lymphotoxin-α and type 1 TNF receptor in the establishment of follicular dendritic cells from non-bone marrow-derived cells.
To facilitate comparisons within bone marrow type MSCs or non-bone marrow type MSCs, we additionally chose vertebral bone marrow and pulmonary tissue, as the MSCs from these tissues were previously shown to have unique properties23,24.
It is also known that non-bone marrow derived cells resident in skin (keratinocytes, fibroblasts) produce but do not process 31 kD IL-1 beta.
Giambini et al. (2015) suggest using dual-energy CT to isolate bone from non-bone constituents within the matrix.
We also studied a number of non-bone-forming secondary lesions (carcinomas that formed lytic metastases to the skeleton) and found BMP production in most of these tumors.Results.-We found that BMPs were expressed in both bone-forming and non-bone-forming benign musculoskeletal lesions.
Recent evidence has shown it may also have a role to play in preventing non-bone-related diseases such as Parkinson's, dementia, cancers and inflammatory diseases.
We therefore used TRAP secretion as a marker to detect resorptive behavior on non-bone substrates.
