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These changes are localised specifically to NuRD target genes, but not to the non-targets assayed.
Note that this estimate includes additional non-assay sources of variation because of the immediate separation after centrifugation.
We used three non-cellular assays to measure the DPPH radical scavenging capacity, oxygen radical absorbance capacity (ORAC) and total phenol content (FC assay) [ 58].
Motile males that were non-Dpy were assayed from plates that segregated DpyUnc and Unc.
The Chemical Ligation Dependent Probe Amplification assay (CLPA) is a Non Enzymatic Assay Technology (NEAT) based on a non-enzymatic oligonucleotide probe ligation method [25].
SOD activity was assayed using non-denaturing PAGE gels, essentially as described (Gratão et al. 2015).
Lactulose to mannitol ratio (L/M) in urine is used as a non invasive assay to measure intestinal permeability.
Non radioactive assay for Akt used similar protocol with only the radioactive ATP being replaced by 200mM of cold ATP and Gsk3β being used as substrate.
Standard gates were used in 90% of sample assays, non-standard gates used in 10% sample assays.
In non-radioactive binding assays, GST fusion protein-coupled Sepharose beads were incubated with HEK293 total extracts in MTPBS.
These assays non-specifically detect IgG, IgA, and IgM antibodies against PF4/heparin.
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