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Non adherent cells were then removed by washing with PBS.
The analysis was performed on non adherent macrophages [15].
The medium was collected and cleared of non adherent cells.
Non-adherent cells were collected and plated onto non adherent plastic Petri dishes in the same EB medium.
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Initially non-adherent patients received additional adherence support.
By extensive washing, the remaining non-adherent cells were removed.
However, many patients are non-adherent.
Reasons for non-adherent behaviour are not fully understood.
Seeding freshly isolated HSPCs adherent (AT) and non-adherent (SN) cells were found.
This medium could aggregate even non-adherent materials.
Following overnight incubation, non-adherent cells were removed by replacement with fresh culture medium.
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