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The scanning protocol for the Cirrus OCT consisted of the standard five-line protocol, with a nominal scanning length of 6 mm (512 A-scans) without averaging, while the protocol for the Heidelberg Spectralis was a horizontal line of 30 degrees (768 A-scans, 8.7 mm) and the averaging of 100 raw B-scans.
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The nominal scan time was 3 min and 45 s.
Nominal scan times were 4 5.5 minutes (depending on gating parameters), with navigator efficiencies typically ranging from 40 to 60% resulting in total scan times of 6.5 13 min.
AFM measurements were performed with a Nanoscope V multimode (Bruker, Santa Barbara, CA) with a J-scanner (nominal scan size 130 μm) in Tapping Mode.
In fact, with the introduction of frequency increment, the FDA apparent angle will be different from its nominal beam scanning angle.
Optimization strategies were based on the previous screening of the most important instrumental factors like number of cumulated scans, nominal resolution, mirror velocity and zero filling factor, based on a two-level full factorial design and on the search for the optimum conditions using central composite designs.
MetAlign is another efficient data processing freeware for the pre-processing and comparison of full scan nominal or accurate mass LC MS and GC MS data.
During each one-second full-range FT-MS scan (nominal resolution of 60,000 FWHM, 300 to 2000 m/z), the three most intense ions were analyzed via MS/MS in the linear ion trap.
The internal standard is defined by its (nominal) mass and its scan number (211 Da and scan 1082) that can be readily obtained from the chromatograms.
An alignment of two chromatograms with about 5400 scans each, 500 nominal mass bins, 38 defined anchors and a maximum scan deviation of 10% (about 540 scans to the left and right of the diagonal) using the cosine score as local similarity was calculated in 12 s on a MacBook with 2.4 GHz Core2 Duo processor, using around 500 MB of memory.
The images were recorded on a 2K×2K CCD camera (TVIPS, Gauting, Germany) using a homemade microscope control program for spot scan at a nominal magnification of ×80,000, leading to a final image resolution of 0.22 nm/pixel.
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