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In dilution integrity study, the%accuracy of two and four times diluted sample was to 94.6 and 103.6%of the nominal concentration for LND.
In dilution integrity study, the percentage accuracy of two and four times diluted samples were 98.9 and 100.7% of the nominal concentration for linifanib.
Each reactor received a total of 330 mL of the assigned spiking solution (nominal concentration for AgNO3 and AgNP suspensions = 20 mg Ag L-1).
The results obtained on setting negative responses to zero concentration were all closer to the nominal concentration for the standards.
Calibration curves were linear in the range 0.1-8 mcg/ml and the accuracy quality control samples varied from 90 110%% of the nominal concentration for intraday and interday analysis, with a precision (CV%) of 10% or less.
The determined mean PBMC concentrations deviated by -5.72, 9.16 and -7.29% from the nominal concentration for the validation samples containing 14.2 × 10, 67.5 × 10 and 245 × 10 PBMCs/mL, respectively.
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Analytical data ascertaining nominal concentrations for single exposures and for the mixture are given in Table 1.
The range in r values (0.79−1) indicated a strong linear relationship between measured and nominal concentrations for the spiked samples.
The criteria for acceptance of an analytical run specified that the deviation of the predicted concentrations from the nominal concentrations for at least three-fourths of all calibration standards for an analyte and at least two-thirds of all quality control samples for an analyte be within ±15%.
We maintained the nominal concentration of 18 ppm for subsequent water changes for 180 h (7.5 days).
Blank solvent (acetonitrile or methanol) was spiked with the 14 test compounds to generate a nominal concentration of 1 mg/mL for each compound.
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