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The second network (Figure 2B, Table S4) involves nodes of genes that play an important role in oxidative stress (NOS), endocytosis (clathrin), inflammatory response (AP1), and apoptosis (JUN).
The first network (Figure 2A, Table S3) involves nodes of genes that participate in the inflammatory response (TGFB1) as well as in synaptic functioning (ARRB1 and APLP1).
The third network (Figure 2C and Table S5) involves nodes of genes that serve a role in AβPP processing (Furin), synapse development (MEF2), neuroprotection (NR41), insulin metabolism (IDE), and histones deacetylation (HDAC9).
The fourth network (Figure 2D and Table S6) is built on nodes of genes that participate in the cleavage of fatty acids (PLA2), in calcium signalling (calmodulin) as well as in glutamatergic neurotransmission (GRIN1, GRIN2c).
The fourth down regulated network (Figure 3D; Table S10) is centered on nodes of genes that are playing a role in the inflammatory response (CD3) and oxidative stress (GST).
The first network (Fig. 3A and Table S7) is centered on nodes of genes involved in the morphological and functional integrity of synapses (Homer, Homer I), preservation of cholinergic activity (ESR1), and gene expression (EP300).The second network (Figure 3B and Table S8) shows nodes involved in apoptosis (CDH1) and cell motility (G-actin).
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These common nodes of gene targets strongly suggest a down stream target being regulated by the respective miRNAs.
Transcription factor binding sites (TFBSs) on the DNA are generally accepted as the key nodes of gene control.
This shows that single nodes of gene regulatory networks can be much more complex than they are usually considered.
These 23 genes form the nodes of a gene network which includes the gene-gene associations with known literature reports (grey-colored edges) and predicted implicit gene-gene associations (red-colored edges).
In the case of the TRN, the nodes consist of genes, with their associated promoter regions (PRs) and the transcription factors (TFs) which they code, if any.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com